![Diane](https://www.csuohio.edu/sites/default/files/Diane%20Green%20Web.png)
Diane Jackel Greene
Facilities manager/Administrator
Location: SI 201
Phone: (216)875-9824
Fax: (216)687-6972
Manages shared instrumentation within the Center for Gene Regulation in Health and Disease (GRHD). Responsible for general upkeep, routine maintenance, and quality control of high-end instrumentations.
If you are planning to use advanced fluorescence-based Confocal and DeltaVision microscopes, a BD FACSCanto™ benchtop flow cytometer and/or any other shared equipment in the Center, please e-mail and/or call Diane Greene.
Special features of our Nikon A1Rsi Laser Scanning Confocal Microscope include: High resolution galvano scanner (4096x4096 pixels); High speed resonant scanner (30 fps at 512x512 pixels); Transmitted detector for brightfield imaging and Differential Interference Contrast; Spectral imaging capability (can image GFP and Alexa 488 in same sample); DIC optics for transmitted light imaging; Perfect focus system (eliminates focus drift during live imaging); Motorized XYZ stage with piezo control; Photoactivation, photobleaching, and FRET capable; Environmental chamber.
Special features of our Deltavision Deconvolution Microscope include: Filter sets for imaging of DAPI, FITC, Cy3, and Cy5 or similar fluors; Cooled CCD for high speed, low noise image acquisition; DIC optics for transmitted light imaging; Motorized XYZ stage with piezo control; Workstation for image acquisition and deconvolution (upgraded 2018); Environmental chamber.
![Nikon](https://www.csuohio.edu/sites/default/files/Nikon%20for%20web.jpg)
![DeltaVision](https://www.csuohio.edu/sites/default/files/DeltaVision%20for%20web.jpg)
Nikon A1Rsi confocal microscope DeltaVision deconvolution microscope
![BD FACSCanto](https://www.csuohio.edu/sites/default/files/BD%20FACSCanto%20II%20web.jpg)
![Typhoon 9410](https://www.csuohio.edu/sites/default/files/Typhoon%209410%20web%2001.jpg)
BD FACS Canto II Flow Cytometer Typhoon 9410 imaging scanner
![Typhoon new](https://www.csuohio.edu/sites/default/files/Typhoon%20new%20web.jpg)
![New GRHD lab](https://www.csuohio.edu/sites/default/files/New%20GRHD%20lab%20web.jpg)
Amersham Typhoon 5 Imager Newly renovated GRHD lab
![L90K](https://www.csuohio.edu/sites/default/files/L90K%20web%2001.jpg)
![J2-21](https://www.csuohio.edu/sites/default/files/J2-21%20web.jpg)
Beckman Optima L90K ultracentrifuges Beckman J2-21 centrifuges
![Odyssey](https://www.csuohio.edu/sites/default/files/Odyssey%20web.jpg)
![French press](https://www.csuohio.edu/sites/default/files/French%20press_web.jpg)
Odyssey Fc Imaging System Thermo Electron French press
![Cell culture](https://www.csuohio.edu/sites/default/files/Cell%20culture%20new%20web.jpg)
![LS counter](https://www.csuohio.edu/sites/default/files/LS%20counter%20web.jpg)
New cell culture facility Beckman LS 6500 scintillation counter
![AKTA](https://www.csuohio.edu/sites/default/files/AKTA%20web.jpg)
![Heska](https://www.csuohio.edu/sites/default/files/Heska%20web.jpg)
AKTA Purifier FPLC System Heska Hematology and Element Analyzers